Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2021
  • Volume: 14
  • Issue: 10

Validation UV Spectrophotometric Method to Determine Entrapment Efficiency of Ocular Polymeric Nanoparticle Levofloxacin Hemihydrate

  • Author:
  • Indri Maharini14, Ronny Martien2, Akhmad Kharis Nugroho2, Supanji3, Adhyatmika2
  • Total Page Count: 4
  • Page Number: 5479 to 5482

1Program Doctoral Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

2Departement of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta-55281, Indonesia

3Department of Ophthalmology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta-55281, Indonesia

4Departement of Pharmaceutics, Faculty of Medicine and Health Sciences, Universitas Jambi, Jambi-36361, Indonesia

*Corresponding Author E-mail: ronnymartien@ugm.ac.id

Online Published on 22 February, 2022.

Abstract

Simple, easy, and economical UV spectrophotometric methods have been developed using simulated tear fluid (STF) solvent to determine levofloxacin hemihydrate (LEVH) levels in ocular nanoparticles, as indicated by the % entrapment efficiency. A maximum wavelength of levofloxacin at 288nm, with a 4.0–12.0μg/mL concentration, shows linear results with a correlation coefficient (r) of 0.9997 and an average % recovery of 99.920 –100.804%. The developed method is able to use for the analysis of levofloxacin hemihydrate in nanoparticle preparations.

Keywords

Levofloxacin hemihydrate, Entrapment efficiency, Validation, Simulated tear fluid