Identification and isolation of lupeol and p-sitosterol from iraqi Bauhinia variegata and determination the cytotoxic activity of the hexane extract of its leaves, stems and flowers

Objective: Bauhinia variegata is evergreen small medicinal tree grows all over the world especially in the tropical regions. The aims of the study were detection, isolation quantification and identification of p-sitosterol and lupeol of Iraqi B. variegata stem using various chromatographic and spectroscopic methods, beside the cytotoxic investigation of hexane extract of different aerial parts. Methods: detection by GC mass spectroscopy, isolated by preparative TLC. The identification and the structural elucidation of isolated p-sitosterol and lupeol were performed by ¹H nuclear magnetic resonance and FTIR. While the cytotoxic activity was performed against MCF-7 cells line. Results: Results highlighted that p-sitosterol and lupeol present in Iraqi B. variegata stem that confirmed by different chromatographic and spectroscopic analysis. Also the leaves extract has the superior action on MCF-7 cells followed by stem extract and end up by flower extract. Conclusions: The plant has a good content of both p-sitosterol and lupeol and the good cytotoxic action of aerial parts hexane extract confirm the successful plantation of B. variegata. These isolated compounds are a suitable candidate for further pharmacological study.