Department of Pharmaceutical Quality Assurance, Rajgad Dnyanpeeth's College of Pharmacy, Bhor, Tal. Bhor, Dist. Pune-412 206, Maharashtra.
A new, simple, accurate, precise and reproducible UV-Spectrophotometric method is being developed for the simultaneous estimation of ibuprofen and thiocolchicoside in tablet dosage form for first time. The stock solutions were prepared in methanol. The λmax for Thiocolchicoside and Ibuprofen were 256nm and 228nm respectively. The Thiocolchicoside, Ibuprofen obeyed Beer's law in concentration range of 0.4–1.2μg/ml and 0.5–2.5μg/ml respectively. Results of analysis of absorbance ratio method were analysed and validated for various parameters according to ICH guidelines for accuracy, precision, linearity, robustness, LOD and LOQ. The proposed method is highly sensitive, precise and accurate, therefore can be used for intended purpose.
Thiocolchicoside, Ibuprofen, Absorbance Ratio Method, Validation, ICH
