Effect of Bryophyllum pinnatum leaves Ethanol extract in TNF-α and TGF-β as candidate therapy of SLE in pristane-induced SLE BALB/c mice model

SLE is an autoimmune disease that has very complex pathogenesis. SLE patients have more sensitive B cells and reduced regulator T cell activity. The increase in activityof B which has play a role in the absorption and presentation of autoantigens to T cells. B cells and T cells will also affect the production of cytokines such as TNF α and TGF-β. We used forty female BALB/c mice, aged 6 until 8 weeks old received a single intraperitoneal injection of 0.5 cc pristane for lupus induction. At 16 weeks after injection, pristane-induced lupus mice were divided into four groups based on the doses received by intragastrically: 10.5mg/kg/day, 21 mg/kg/day, and 42mg/kg/day for 12 weeks. At 38 weeks after injection, all of mice were assessed for inflammation marker. Study results showed that percentages of maturation of B cells, TNF-α, were decreased (p<0.05) and percentages of TGF-β were increased (p>0.05) in Bryophyllum pinnatum leaves ethanol extract-treated mice. Ethanol extract of Bryophyllum pinnatum can inhibit the maturation of B cells so that it can decrease the auto antigen presentation rate in T cells. Bryophyllum pinnatum can also reduce TNF α levels that have been pro-inflammatory agents, whereas Bryophyllum pinnatum can increase TGF-β levels which have the role of anti-inflammatory agent.