Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, Gandhi Institute of Technology and Management (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, India.
*Corresponding Author E-mail: kalyani.lingamaneni@gmail.com
Online published on 30 April, 2021.
Pterostilbene belongs to phytoalexins group and it is chemically trans 3, 5-dimethoxy-4’-hydroxy-trans-stilbene. Pterostilbene is used for the treatment of diabetes, cancer and fungal infections. A new stability indicating chromatographic method has been developed for the quantification of Pterostilbene and the method was validated. The chromatographic study was studied using Shimadzu Model CBM-20A/20 Alite UFLC system with Sunfire C18 column and PDA detector. A mixture consisting of 0.1M ammonium formate and methanol (15: 85, v/v) with flow rate 0.8 mL/min. Linearity was observed over the concentration range 0.05–150 μg/mL (R2 = 0.9999) with linear regression equation y = 207961.521x-16083.7544. The LOD and LOQ were obtained to be 0.01569 μg/mL and 0.04782 μg/mL respectively. Pterostilbene drug solutions were subjected to stress conditions such as acidic, alkaline, oxidation and thermal degradations. The method was validated and can be applied for the quantification of Pterostilbene pharmaceutical formulations.
Pterostilbene, RP-UFLC, Stability-indicating, Validation, ICH guidelines
