In vitro Urolithiasis activity of Thinopyrum intermedium methanolic extract on calcium oxalate crystals prepared by Precipitation method

In case of developing countries like India incidence rate kidney stones were significantly increased due to the life style modifications from the last two decades so the present study aimed to evaluate invitro urolithiasis activity of Thinopyrum intermedium on experimentally prepared calcium oxalate crystals. calcium oxalate crystals were prepared by homogenous ppt method by using calcium chloride and sodium oxalate. the crude extract was prepared by the simple maceration with methanol 1: 3 ratio and the solvent was evaporated by rotary evaporator and two doses of extract selected i.e 10mg and 20mg and compared against standard cytosine all were assayed against calcium oxalate crystals which were incubated in semipermeable membrane with sulphuric acid. the results were 68.02% (10mg), 72.41% (20mg) and 90% and we conclude that the Thinopyrum intermedium was showed significant effect of urolithiasis.