Study on the ability of producing of conjugated Linoleic acid of Lactobacillus fermentum A01 isolated from human digestive tract

The most abundant isomers of conjugated linoleic acid (CLA) are cis-9 trans-11 (c9t11) and trans-10 cis-12 (t10c12) that involved in a number of health aspect. At present, lactic acid bacteria was thought to produce CLA from LA. Therefore, the study was conducted to determine the conjugated linoleic acid (CLA) producing ability of Lactobacillus fermentum A01 (L. fermentum A01) in grape seed oil and linoleic acid. The CLA was detected on thin layer chromatography and gas chromatography-mass spectrum (GC-MS) analysis after converting to fatty acid methyl esters. L. fermentum A01 could produce CLA by thin layer chromatography (TLC) analysis. Further investigation revealed L. fermentum A01 cultured in medium containing linoleic acid (MRS-LA) for 36 h could produce 9, 11 di-ene of C18 at the retention time of 30.299 min with identical percentage (0.67%). Moreover, there were many products produced as 9, 12; 9, 15; 11; 10; 14-C18 with higher percentage in MRS-LINO and MRS-Oil. This study was the preliminary report of L. fermentum A01 in the ability of converting LA to CLA.