Screening of effective formulation techniques for Designing and Fabrication of terbinafine hydrochloride ethosomes

The present investigation aimed to design and fabricate terbinafine HCl (TH) loaded ethosomes for enhancement of transdermal delivery in the management of fungal diseases. The TH loaded ethosomes were explored initially by the selection of appropriate method amongst reported one and further optimized for best amongst the investigated. In present study, TH loaded ethosomes were prepared using cold method, ethanol injection method, and mechanical dispersion using rotary evaporator method and assessed for vesicle size, drug entrapment, zeta potential, and polydispersity index (PDI). The mechanical dispersion using thin film hydration method (vesicle size 126.6±1.55nm, entrapment efficiency 82.10±0.25%) was selected as an appropriate for development of TH ethosomes. Ethosomes prepared using thin film hydration method was optimized on the basis of concentration of TH: PL 90H (1: 2, 1: 4, 1: 6 and, 1: 8), hydroethanol 20–45%, volume of ethanol, stirring speed and sonication time. The comprehensive analysis revealed that concentration of PL 90H (TH: PL 90H ratio 1: 2, 1: 4, 1: 6), hydroethanol 20–40% w/v with 10 mL ethanol for film formation following stirring speed 60 rpm, 100 rpm for rehydration of film and 30 min ultra-bath sonication were desired parameters for development of TH ethosomes. The aforementioned optimization results observed in the limit for vesicle size 105.56±2.77nm to 178.71±2.07 nm, entrapment efficiency 63.04±0.67% to 89.52±0.87% with-31 mV to-24 mV zeta potential indicating stability of vesicles. The recorded results indicate that mentioned optimization aspects can be useful for development of drug loaded ethosome with factorial design approach.