Formulation and evaluation of Novel Herbal ointment for the treatment of fungal infection

Clove (Eugenia caryophyllus) and orange peel oil are most valuable spices that have been used for centuries for many medicinal purposes. It is effective in reducing fungal infection such as athlete's foot. (a contagious fungal skin infection caused by a species of trichophyton or epidermorphyton (tinea pedis) that usually affects the foot). Pre formulation studies were done for antifungal activity of clove and orange oil. (clove oil and orange oil mixture was taken and soaked in a sterile disc and those disc were placed in the medium and incubated, zone of inhibition was identified). FTIR was done for the determination of constitute present in the orange oil and clove oil. there were no additional peaks were found when both the oil was mixed for preparation of formulation it was confirmed with the IR interpretation. The herbal ointment was formulated by using clove oil and orange peel oil with simple ointment bases. The extracts of orange peel oil and clove oil were incorporated into simple ointment base by fusion method. The physiochemical parameters such pH, viscosity, spreadability, washability, homogeneity was evaluated. The optimized formula (F1 &F3) was concluded based on evaluation parameters.