Development, optimization, and validation of a green spectrofluorimetric method for the determination of moxifloxacin using an experimental design approach

Green chemistry is an emerging field concerned with safe practices in chemical method development. The purpose of the study is to develop a sensitive, environmentally friendly spectrofluorimetric method by studying and optimizing variables affecting the native fluorescence intensity of Moxifloxacin using experimental design approach. The analysis was divided into three phases: studying and screening the critical factors using 2-level full factorial design, optimization using central composite design and validation according to International Conference on Harmonization (ICH) guidelines. The optimal experimental conditions obtained from this study were 0.05 M phosphate buffer with an adjusted buffer pH of 9.7 and a temperature of 7.4°C±0.5, providing a sensitive measurement of MOX. The validated method providing to be linear over a range of 5-40ng/mL (r= 0.9999), precise, accurate, and robust (demonstrated by the Plackett-Burman design). It can be concluded that the validated method can be safe, optimum, and environmentally friendly alternative for the analysis of MOX