Preparing a new chromatographic support from local syrian clay and using it in thin layer chromatography for simultaneous determination of Paracetamol, Caffeine and Aspirin in tablets

A new chromatographic support was prepared from Local Syrian Clay (Bentonite), using thermal and acid treated Clay (B_500AW) for utilizing it in thin layer chromatography (0.25mm thickness) to separate and determine of Paracetamol, Caffeine, and Aspirin in raw material and in tablets. The separation carried out using mobile phase consisted of Cyclohexane-Chloroform-Methanol-acetic acid (14: 5: 0.25: 0.75) v/v. The specific surface area of treated bentonite was 45m²/g. Quantification was carried out densitometerically at λ = 250nm for Paracetamol, λ = 275nm for Caffeine, and at λ = 200 nm for Aspirin. The retardation factors (R_f) of Paracetamol, Caffeine, and Aspirin were 0.10, 0.21, and 0.40 respectively. Calibration curves were obtained in the concentration ranges of 5.0–40.0μg/spot, 2.0–16.0μg/spot and 3.0–24.0μg/spot for standard solutions of Paracetamol, Caffeine and Aspirin respectively. The New Prepared Chromatographic Thin Layers were successfully applied for analysis of commercial dosage forms (tablets) containing the drugs with average recovery 98.33–101.83% with RSD not more than 3.86%