1Dept. of Anatomy, Manipal Tata Medical College, Jamshedpur, Manipal Academy of Higher Education, Manipal576104, India.
2Dept. of Anatomy, Ras Al Khaimah College of Medical Sciences, RAK Medical and Health Science University, Ras Al Khaimah, UAE.
3Dept. of Physiology, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, 576104, India.
4Dept. of Biochemistry, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, 576104, India.
5Dept. of Anatomical Sciences, St. Matthew's University School of Medicine, Grand Cayman, Cayman Islands.
6Dept. of Physiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal576104, India.
*Corresponding Author E-mail: anjaneyulu.konuri@manipal.edu
Online published on 3 June, 2021.
As impairment of memory and ischemic brain injury is more common in female of menopausal age, the current study aims to evaluate the protective role of fenugreek seed extract and choline-Docosahexaenoic acid (DHA) on cognitive behavior, oxidative stress and neurodegeneration in ovariectomized-cerebral ischemic rats. Cerebral ischemia was induced by bilateral common carotid artery occlusion (BCCAO) surgery to ovariectomized adult female wistar rats. Fenugreek, choline-DHA and combination of fenugreek with choline-DHA was supplemented for 30days after ovariectomy and continued for 14 more days after BCCAO surgery. Effect of this supplementation on learning and memory, brain oxidative stress and neurodegeneration in the CA1, CA3 hippocampal sub-regions were analyzed. Ovariectomized-cerebral ischemic rats demonstrated learning and memory impairment when subjected to passive avoidance task. Further, these rats also had increased brain oxidative stress, and neurodegeneration in CA1, CA3 sub-regions of hippocampus. Conversely, combined supplementation of fenugreek with choline-DHA to ovariectomized-cerebral ischemic rats exhibited a significant improvement in their learning and memory abilities, with reduced oxidative stress and neurodegeneration in CA1 and CA3 sub-regions of hippocampus
Fenugreek, Choline, DHA, Learning, Memory, Neurodegeneration, Bilateral Common Carotid Artery Occlusion, Ovariectomy
