1School of Biotechnology, International University, Vietnam.
2Vietnam National University-Ho Chi Minh City, Vietnam.
3Faculty of Nautral Sciences, Thu Dau Mot University, Vietnam.
*Corresponding Author E-mail: nhktu@hcmiu.edu.vn
Online published on 3 June, 2021.
Lactic acid bacteria play a vital role in biosynthesis of γ-aminobutyric acid (GABA) in the presence of glutamic acid-major substrate for the process. In recent study, Lactobacillus fermentum A01 (L. fermentum A01) isolated from various sources in Vietnam were screened for bacteria strains with high efficiency in GABA formation. L. fermentum was cultured in MRS broth containing 25 mg/mL monosodium glutamate (MSG), at pH of 6.5 and incubated at the optimal conditions (37ºC, for 24, 48, 72 h). After extraction and thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis, L. fermentum A01 showed the GABA yield about 1.34 mg/g in dried supernatant, suggesting L. fermentum A01 to be a promising GABA producer for food and pharmaceutical applications
Gamma-aminobutyric acid (GABA), Glutamate, Lactobacillus fermentum, Optimization, High performance liquid chromatography
