N-(chlorobenzyl) formamide as an antituberculosis agent from multicomponent reaction synthesis

Tuberculosis (TB) is an infectious disease caused by M. tuberculosis. A new series of four benzylformamide with chloro substitution were synthesized by multicomponent reaction method and screened for antituberculosis activity against Mycobacterium tuberculosis H37Rv strain. This study was based on amidoalkylation reaction of aromatic-aldehyde, formamide and formic acid at 150^oC to give a good yield of benzylformamide. Target compound were isolated, purificated, identified and characterized by GC-MS, FTIR, ¹H-NMR, and ^1,3C-NMR then the antituberculosis activity was examined by Microplate Alamar Blue Assay (MABA) and Middlebrook 7H9–7H11 medium in concentration series of 1000 - 1,954 μg/mL, and isoniazid as a positive control. Among the three derivative, N-(2,4-dichlorobenzyl) formamide has the most potential antituberculosis activity up to 500.0 L/mL.