Uv spectrophotometric method development and validation of darunavir in bulk and solid dosage form

A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the determination of Darunavir in Tablet dosage form with 0.1N HCl.

The method is based on the formation of a colorless complex. The UV spectrum of Darunavir in 0.1N HCl showed maximum wavelength at 298nm. Beer's law is valid in the concentration range of 10–60μg/ml. this method was validated for linearity, accuracy, precision, assay, ruggedness and robustness.

The method has demonstrated excellent linearity over the range of 10–60μg/ml with the regression equation y=0.0113x+0.0098, and regression coefficient i.e. r²=0.9992 moreover, the method was found to be highly sensitive with LOD (1.85μg/ml) and LOQ (5.62μg/ml).

Based on the results the proposed method can be successfully applied for the assay of Darunavir in various tablet dosage forms.