1Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
2Doctoral Program in Mathematics and Natural Sciences, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia
3Department of Chemistry, Myitkyina University, Myitkyina, Myanmar
4School of Environmental Science and Management, University of the Philippines Los Banos, Los Banos, Philippines
5Management and Science University, Shah Alam, Malaysia
6Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Indonesia
7Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
*Corresponding Author E-mail: teguhharisucipto@staf.unair.ac.id
Online published on 25 August, 2021.
Dengue is a major mosquito-borne disease that currently has no effective antiviral or vaccine available. Recently, Indonesia is one of the largest countries in the dengue-endemic region, with a total population of more than 250 million. In the present study, the antiviral activity of P. merkusii stem bark and cone were evaluated against dengue virus type-2 (DENV-2; NCBI accession number: KT012509) isolated from Surabaya, Indonesia. We revealed that P. merkusii stem bark and cone inhibited DENV-2 in Vero cells (originally from African green monkey kidney) with ICs0= 140.63 μg/mL and 73.78 μg/mL, CCs0= 89.65 μg/mL and 249.5 μg/mL, SI= 0.64 and 3.38, respectively. The findings presented here suggest that P. merkusii stem bark and cone exerts potent antiviral activity against DENV-2. Hence, P. merkusii stem bark and cone are potent to inhibit DENV-2 and should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV-2.
Antiviral, Dengue, Pinus merkusii, Vero Cell
