1Department of Bio-Engineering, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600117
2ARMATS Biotek Training and Research Institute, Guindy, Chennai, Tamil Nadu - 600032
*Corresponding Author E-mail: meenambiga.se@velsuniv.ac.in
Online published on 25 August, 2021.
The presented work represents phytochemical analysis, Antioxidant assay and Antimicrobial activity of Bhut jolokia pepper (Capsicum chinense Jacq) extracted by ethanol as solvent. The ethanolic extract of Bhut jolokia pepper showed the presence of terpenoids, steroids, saponins and flavonoids. Antimicrobial assay was done with varying concentration (250–1000μg/ml) of pepper extract using tetracycline as control by well diffusion method, the extract at 750μg/ml shown best inhibition zone and Staphylococcus aureus showed the highest zone of inhibition at all concentration compared to other bacteria species with maximum zone of inhibition of 27mm. The DPPH scavenging assay for antioxidant activity at 517nm showed positive activity for scavenging, scavenging increased with the concentration of extract. Thus, Bhut jolokia could be effective in treating diseases caused by Staphylococcus aureus from antimicrobial assay result.
Bhut jolokia, Phytochemical analysis, DPPH scavenging assay, Antimicrobial assay, Staphylococcus aureus
