Uv spectrophotometry method development and validation of luliconazole in bulk and formulation

A new, simple, economical, sensitive, precise and reproducible UV visible spectrophotometry method was developed for the estimation of luliconazole in pure form and pharmaceutical formulation as per ICH guidelines.

A UV spectrophotometry method has been developed using methanol and water as solvent to determine the luliconazole in bulk and pharmaceutical dosage formulation. The Xmax of luliconazole in methanol and water was found to be 297nm.

The drug was proved linear in the range of 3–15μg/ml and exhibited good correlation coefficient (R²= 0.9993) and excellent mean recovery (98–99%). The % RSD for intra-day and inter-day precision was found to be 1.051288 and 1.138658 respectively. The LOD and LOQ of Luliconazole was found to be 1.1168μg/ml and 3.3845μg/ml respectively. This method was successfully applied to luliconazole content in marketed brands and results were in good agreement with the label claims.

The method was validated for linearity, precision, repeatability and reproducibility. The obtained results proved that the method can be employed for the routine analysis of luliconazole in bulks as well as in commercial formulations.