1Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman19328, Jordan
2Department of Pharmacy, Faculty of Pharmacy. The University of Jordan, Amman, Jordan
3Department of Medical Analysis, Mutah University, Mutah, Karak, 61710, Jordan
4Division of Anatomy, Biochemistry, and Genetics. Faculty of Medicine and Health Sciences, An-Najah National University, Nablus
5Department of Biology, Mutah University, Karak, Mutah, 61710Jordan
6Department of Medical Laboratory Sciences, Faculty of Pharmacy, Jadara University, Irbid, Jordan
7Department of Biological Sciences, Faculty of Science. The University of Jordan, Amman, Jordan
*Corresponding Author E-mail: m.alqaraleh@ammanu.edu.jo
Online published on 25 August, 2021.
Branched chain amino acids (BCAAs) can be tightly connected to metabolism syndrome (MetS) which can be counted as a metabolic indicator in the case of insulin resistance (IR). The aim of this study was to assess the potential role of these acids under oxidative stress. Material and
the in vitro antioxidant activity of BCAAs was assessed using free radical 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging assays. For further check, a qRT-PCR technique was made for detection the extent of alterations in gene expression of antioxidative enzymes (catalase and glutathione peroxidase (Gpx)) in lipopolysaccharides (LPS)-induced macrophages RAW 264.7 cell line. Additionally, BCAAs antioxidant activity was evaluated based on plasma H2O2 levels and xanthine oxidase (XO) activity in prooxidative LPS-treated mice.
Different concentrations of BCAAs affected on DPPH radical scavenging activity but to lesser extent than the ascorbic acid. Besides, BCAAs obviously upregulated the gene expression levels of catalases and Gpx in LPS-modulated macrophage RAW 264.7 cell line. In vivo BCAAs significantly minimized the level of plasma H2O2 as well as the activity of XO activity under oxidative stress.
our current findings suggest that BCAAs supplementation may potentially serve as a therapeutic target for treatment of oxidative stress occurs with atherosclerosis, IR-diabetes, MetS and tumorigenesis.
BCAAs, Catalase, DPPH, Glutathione peroxidase, Xanthine oxidase
