Marine Biotechnology Laboratory, Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India
*Corresponding Author E-mail: kvbhaskararao@vit.ac.in
Online published on 1 October, 2021.
Protease constitutes the major group of catalytic enzymes which is involved in hydrolyzing peptide bond of proteins. Marine sediment sample were collected and protease producing bacterial isolates were identified by using casein as a substrate. The organisms were characterized by biochemical test and identified as Bacillus sp. In order to check for the production of protease enzyme, quantitative protease assay and Lowry's method of protein estimation was carried out. The crude extract of protease was subjected for blood stain removal activity and the enzyme proved to be efficient which removed the stain in 15 min. The purpose of the current study is to isolate, identify, characterize and to carry out applications of protease enzyme from marine bacteria isolated from mangrove sediment samples.
Protease, Blood stain removal, Protein estimation, Marine bacteria
