1Industrial Microbiology, Dept., Directorate of Agricultural Research Science and Technology, Baghdad- Iraq
2Dept. of Pharmacy, Faculty of Pharmacy, Middle East University, Amman, Jordan
3Faculty of Vet. Med., University Malaysia, Kelentan
4Dept. of Medical laboratory Sciences, Faculty of Allied Medical Sciences, Al Ahliyya Amman University, Amman, Jordan
5Faculty of Medicine, Lincoln University College, Malaysia
*Corresponding Author E-mail: imad@lincoln.edu.my
Online Published on 22 February, 2022.
In this study we examined P. betle antibacterial property on two bacterial species namely: Streptococcus agalactiae and Escherichia coli. The extract of P. betle was prepared by mixing 2 gm of P. betle powder with 20 ml of 70% ethanol. Then, the solvent was evaporated by using vacuum evaporator and the residue was freeze dried and stored at −20 ºC. The P. betle extract divided into three different concentrations that are 50 mg/ml, 75 mg/ml and 100 mg/ml. The results revealed that the leaves of Piper betle have many benefits including antibacterial activity. The three concentrations showed an effective zone of inhibition against S. agalactiae and E. coli using Kirby-Baeur method. In addition, the minimal inhibitory concentration (MIC) confirms the effectiveness of P. betle. This study findings indicated that P. betle extract can be a new source of antibacterial agent and an alternative antibacterial for control of S. agalactiae and to certain extant against E. coli infections.
Streptococcus agalactiae, Piper betle, Antibacterial agent's, MIC
