1Doctoral Program, Faculty of Medicine, Brawijaya University, Malang65145, Indonesia
2Plastic, Aesthetic and Reconstruction Consultant, Saiful Anwar General Hospital, Malang65111, Indonesia
3Pharmacology Department, Faculty of Medicine, Brawijaya University, Malang65145, Indonesia
4Clinical Pathology Department, Faculty of Medicine, Brawijaya University, Malang65145, Indonesia
5Faculty of Medicine and Health Sciences, State Islamic University of Malang, Malang65144, Indonesia
*Corresponding Author E-mail: wihastyokohyl@gmail.com
Online Published on 22 February, 2022.
This study aims to identify the potential of papain as a candidate for the treatment modality for abnormal scars via in silico studies.
We determined the potential mechanism of the process of collagen degradation by papain by investigating its cleavage site-specificity and identifying human papain-like enzymes that have comparable biological activity in degrading collagen in the extracellular matrix using Merops, Bioedit, String DB and Cytoscape software.
Papain targets QQ_D (Glutamine-Glutamine Aspartic acid) motif for degradation while collagen only has QQ (Glutamine-Glutamine) motif. Additionally, the homology result showed that Cathepsin B has a closer relationship with papain compared with another candidate, Cathepsin K.
Papain is a potential therapeutical modality candidate in degrading collagen in abnormal scars with an indirect mechanism as indicated by its cleavage site-specificity and its relationship with Cathepsin B, which degrades collagen via ubiquitin (UBC) proteasome.
Abnormal scar, Cathepsin, In silico, Papain enzyme
