1Laboratory of Animal Product Technology, Faculty of Animal Husbandry, Universitas Brawijaya, Malang, Indonesia, 65145
2Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia, 65151
3Animal Clinic, Faculty of Veterinary Medicine, University of Brawijaya, Malang, Indonesia, 65151
4Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia, 65151
*Corresponding Author E-mail: lilik.eka@ub.ac.id
Online published on 1 March, 2023.
The poultry industry was an industry that supported the nutritional needs of all individuals and the world's food needs. Coffee was a natural ingredient consisting of antioxidant molecules such as Chlorogenic Acid (CGA). This research using ISA brown chicken layer, which divided into five groups such as healthy chicken (control group (C-)), chicken infected with Salmonella enteritidis 108 CFU/ml (control group (C+)), chicken infected with S. enteritidis 108CFU/ml and extract Coffee consecutive 500, 1000 and 1500 mg/kg body weight (BW) group (T1, T2, and T3). The relative level of TCD4, CD8, and CD45 cells was performed with One way ANOVA test. The research results showed that the relative level of TCD4+, TCD8+ and TCD45+ cells in T1 and T2 groups was not significant with the negative control group. The conclusion is that extracting Coffee in the T1 and T2 groups with a 500-1000mg/kg BW dose could regulate immune cell activation in chicken infected by S. enteritidis bacteria.
Chicken, Coffee, Antioxidant, T helper cell, Cytotoxic Cell, Salmonellosis
