Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2022
  • Volume: 15
  • Issue: 11

Chlorogenic acid fractionation in robusta green bean extract as a combination agent of dental pulp stem cells in periodontal tissue engineering

  • Author:
  • Sandra Sari Desi1,*, Neira Sakinah1, Nuri2, Enny Suswati3, Retno Widyowati4, Ernie Maduratna5
  • Total Page Count: 6
  • Page Number: 5005 to 5010

1Department of Periodontic, Faculty of Dentistry, University of Jember, Jember, Indonesia

2Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Jember, Jember, Indonesia

3Department of Microbiology, Faculty of Medicine, University of Jember, Jember, Indonesia

4Department Fitokimia & Farmakognosi, Faculty of Pharmacy, University of Airlangga, Surabaya, Indonesia

5Department of Periodontic, Faculty of Dental Medicine, University of Airlangga, Surabaya, Indonesia

*Corresponding Author E-mail: desi_sari.fkg@unej.ac.id

Online published on 3 March, 2023.

Abstract

Robusta coffee beans contain very high chlorogenic acid. In the last decade, chlorogenic acid was developed as an adjunct in stem cells to enhance the anti-inflammatory and antioxidant properties of stem cells when used as a therapeutic agent. Chlorogenic acid can increase proliferation and migration and inhibit the production of pro-inflammatory cytokines in stem cells thereby increasing the ability of stem cells to regenerate tissue.

To analyze the levels of chlorogenic acid in robusta coffee bean extract which can be used as a combination agent for Dental Pulp Stem Cells (DPSC) in periodontal tissue engineering therapy.

Robusta coffee bean extract was obtained from the Coffee and Cocoa Research Center, Jember Regency which was processed and processed using the fractionation method. This study used DPSC with the extraction of premolar teeth of orthodontic patients. The toxicity test was performed on the coffee extract 0.0625%; 0.125%; 0.25%; 0.5% to determine the biocompatible concentration of DPSC.

The highest fractionation and measurement of chlorogenic acid content obtained was 30.49%. Robusta coffee bean extract with a chlorogenic acid content of 30.49% at concentrations of 0.125% and 0.0625% did not cause toxicity to DPSC.

Chlorogenic acid in robusta coffee bean extract concentrations of 0.125% and 0.0625% are relatively biocompatible as a combination agent for DPSC in periodontal tissue engineering therapy.

Keywords

Robusta coffee beans, DPSC, Periodontal engineering, Periodontitis, Regeneration