1Dental Regenerative Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
10Oral and Maxillofacial Surgery Resident, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
2Orthodontics Department Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
3Graduate Student of Dental Health Science, Universitas Airlangga, Surabaya, Indonesia
4Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
5Dentomaxillofacial Radiology Department Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
6Oral Biology Department Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
7Malaysian Armed Forces Dental Officer, 609 Armed Forces Dental Clinic, Kem Semenggo, Kuching, Sarawak, Malaysia
8Undergraduate Student, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
9Graduate Student of Clinical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
*Corresponding Author E-mail: alexander.patera.nugraha@fkg.unair.ac.id
Online published on 3 March, 2023.
Oral squamous cell carcinoma (OSCC) is an oral epithelial neoplasm with worldwide prevalence of 95% among all oral malignancies. Therapy of OSCC include radiation, chemotherapy, and surgery but causes subsequent cell death, ineffective long-term therapy, and infection risk at surgical site. Curcumin-quercetin is flavonoid derived from Curcuma longa and Allium cepa that can induce apoptosis and inhibit cells metastasis. Combination of curcumin-quercetin has low solubility hence encapsulated by nanoparticle is necessary. Nanostructured lipid carriers (NLCs) can enhance loading capacity and improve stability of curcumin-quercetin, biocompatible, and enhance site-specific targeting efficiency. Administration of combination of curcumin-quercetin loaded nanostructured lipid carrier by injection targeting OSCC cell.
To analyze the potency of curcumin-quercetin loaded NLCs as OSCC therapy by downregulating AKT/PI3K signaling pathway.
Curcumin-quercetin loaded NLCs are injected into the body then reach the target cells. This combination later would be encapsulated releasing curcumin-quercetin. Curcumin-quercetin suppresses the AKT/PI3K signaling pathway which is considered as strong cell cycle inductor, inhibitor of pro-apoptotic proteins expression, and has a pivotal role in OSCC metastasis. Inhibition of the pathway decreases the expression of cyclin A/D/E and manifests in the G1/G2/M phase arrest. This condition accompanied by p53 and FOXO-1 expression leads to apoptosis. Downregulating of this pathway decreases NF-kB expression then the expression of MMP-2/9 is lowered and manifests in inhibition of extracellular matrix degradation. This condition leads to migration and metastasis inhibition.
Curcumin-quercetin loaded NLCs is potential for OSCC therapy by downregulating AKT/PI3K signalling pathway.
Herbal, Medicine, Cancer, Non-communicable disease, Dentistry
