Physicochemical properties and Structural Characterization of Chitosan Synthesized from Rare Spined Murex, Murex trapa (Roding, 1798) Shell Waste

Chitosan is derived from Murex trapa as the starting source by the process deacetylation of chitin, which is carried out for 6 hours using 40% NaOH at 90ºC. The yield (17%) and physiochemical properties like ash (0.954%), moisture content (4.2%), and solubility, degree of deacetylation (73), fat binding capacity (252%), and water binding capacity (280%) were indicated the M. trapa is a substantial alternate source of chitosan. Fourier transforms infrared spectroscopy (FT-IR) analysis shows characteristic peaks of OH at 3450cm^-1 and amine at 1660cm^-1, X-ray diffraction (XRD) analysis indicated two vital characteristic peaks 10° and 20° at (2θ). Scanning electron microscope (SEM) was used to determine surface morphology of isolated chitosan. Also, Thermogravimetric analysis (TG/DTA) was employed to characterize the thermal stability of M. trapa chitosan. Procoagulant ability, plasma recalcification time assays and minimum bactericidal activity confirmed the hemocompatibility and antibacterial activity of the prepared chitosan. The isolated chitosan can be considered as a potent anticoagulant and antibacterial drug in future.