1Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia
2Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia
3Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah, Purwokerto, 53182, Indonesia
*Corresponding Author E-mail: Budi-u-2@fkh.unair.ac.id
Online published on 13 June, 2022.
Naringin, a bioflavonoid naturally found in citrus species, has been exert as promising anticancer activities. However, naringin mechanism as anticancer still remain enigmatic. This study, WiDr cell lines as human colon cancer cell in vitro were used to evaluate anticancer effect of naringin. Naringin (10, 20, 40, 60, 80µg/mL) treated on WiDr cells to evaluate IC50 value by MTT assay, then continued with analyzed caspase-3 expressions (IC50, 2IC50) as apopotosis marker. This study reported that naringin inhibit the growth of WiDr cell with IC50 value is 63.14µg/mL. The caspase-3 expressions on 2IC50 is higher (74.66%) than IC50 (56.66%). These results indicate that naringin has properties of inhibting cell growth and inducing caspase-3 expressions on WiDr cells. It is a potential compound to be developed as a chemopreventive agent for colon cancer.
Naringin, Colon cancer, Caspase-3, Apoptosis
