A simple and rapid RP-HPLC method for simultaneous estimation of paracetamol and pamabrom in combinational dosage form

The bioanalytical evaluation of paracetamol and pamabrom study had been developed using the RP-HPLC method. The elution was done by the mobile phase containing ammonium formate and methanol. The separation was done by using the Hibar C18 column. The flow rate was adjusted to 1.0 ml/min with an injection volume of 20 μl. The wavelength of 268 nm was overlaid for analytical purposes. A volume of 25 μg/ml of internal standard (furosemide) was injected. Paracetamol and pamabrom showed retention time at 7.05 min and 10.02 min with no interference peak from internal standard showing a retention time at 11.97 min. The values of the limit of detection (LOD) and limit of quantification (LOQ) for paracetamol and pamabrom was found to be 0.03 μg/ml and 0.015 μg/ml; 0.1 μg/ml and 0.05 μg/ml respectively. The developed method was rapid, simple, accurate, precise, robust and selective. This method can be used for the simultaneous estimation of the solid oral dosage forms containing paracetmol and pamabrom in biological sample.