UV Spectrophotometric Method Development and Validation for the Determination of Ascorbyl Palmitate in Bulk and its Pharmaceutical Dosage Form

A simple, economical, accurate, precise and less time-consuming UV spectrophotometric method has been developed and validated for estimation of Ascorbyl palmitate in bulk and pharmaceutical formulations. In this method, Ascorbyl palmitate exhibits maximum absorbance (λ max) at 261nm. The drug obeys Beer’s law in the concentration range of 2-12μg/ml. The method was validated as per the International Conference on Harmonization (ICH) guidelines. Drug followed the linearity in the concentration range of 2-12μg/ml with correlation coefficient (R²) of 0.998. The validity of the proposed method was assessed by applying the standard addition technique where the percentage recovery of the added standard was found to be 100% -109.7%. The limit of detection and quantification were calculated to be 0.1200µg/ml and 0.363μg/ml respectively. The proposed method is recommended for routine analysis of Ascorbyl palmitate in bulk and dosage forms in quality control testing laboratories. Since it is rapid, simple, accurate, sensitive and economical.