1Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia
2Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia
3Drug Development Research Cluster, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia
4Human Cancer Research Center, Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia
5Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta, 10430, Indonesia
*Corresponding Author E-mail: yurnadi.kes@ui.ac.id
Online published on 21 February, 2023.
Colorectal cancer (CC) is one type of cancer with a high incidence worldwide. Many therapeutic techniques have been for CC but have not yet yielded satisfactory results. Fish oil has potential as an alternative therapy for CC through its anti-inflammatory effects. Here, we want to investigate that effect on TNF-α expression using omega-3-rich fish oil (FO).
FO was injected into Swiss mice that have been induced (2% in drinking water) with DSS for seven consecutive days. Animals were separated into six groups: normal, negative control, positive control, solvent control, and FO groups (3 and 6 mg/kg body weight/day). All animals were sacrificed, and the colons were collected then stained with anti-TNF-α. The stained sections were subsequently examined with ImageJ based on colour density.
Based on the H-Score of each group, FO 3 mg and 6 mg has significantly decreased the expression of TNF-α compared to the negative control (p=0.001 and p=0.009).
FO administration was able to inhibit the expression of TNF-α on mice's colonic tissue induced with AOM and DSS.
Fish oil, TNF-α, Anti-inflammatory, Colorectal cancer, Mice's colonic tissue
