1Kunwar Haribansh Singh College of Pharmacy, Jaunpur222001 (U.P.), India.
2Faculty of Pharmacy, Integral University, Dasauli, P.O. Bas-ha Kursi Road, Lucknow – 226026 (U.P.), India.
*Corresponding Author E-mail: badarmiracle@gmail.com
Online Published on 23 February, 2023.
Evaluation of toxicity level after acute oral exposure to Lupeol and Naringin combination in rats according to OECD NO.423TG.
Female Sprague Dawley (S.D) rats were used in the study, the experiments was conducted into two groups. One group served as a control and other group recived Lupeol+Naringin combination (2000mg/kg+2000mg/kg body weight).The sign, symptom and mortality were observed for 14 days. Then the parameter was measured for organ weight, haematology, biochemical analysis and histopathology of vital organ (liver, heart, and kidney).
This study clearly indicated that the above combination does not contain any toxicity. The median lethal dose (LD50) of the combination drug is more than 2000 mg/kg body weight and therefore, no any toxicity signs were seen at single dose of 2000 mg/kg body weight without any adverse effects.
The combination drug Lupeol+Naringin did not cause any major toxicity when given orally in rats. During the 14-days research period at 2000 mg/kg b.wt of each compound, no toxic effects were found. This specifically showed that the combination had no toxic effects. Overall, the Lupeol+Naringin combination has been shown to be a safe natural bioactive compound.
Lupeol, Naringin, Acute oral toxicity
