Role of Nacre and Biodentine to Inducing the TGF-β1 in the Dentin Tertiary Formation on the Pulpitis Reversible of Rattus norvegicus

Pulpitis can cause sensitivity and trigger endodontic infections that threaten to lose dentin. Nacre and Biodentine were reported as a stimulator of TGF-β1 expression to induce tertiary dentin formation. To evaluate the ability of Nacre and Biodentine in inducing the TGF-β1 expression of tertiary dentin formation. Thirty male Wistar rats (Rattus Novregicus) were divided into three groups. Haemotoxylin and Eosin staining observed the dentinal bridge formation while TGF-β1 expression was evaluated by immunohistochemistry. Quantitative data were obtained based on the staining score. Kruskal-Wallis tests were used for statistical analysis. Nacre contains Calcium (95.04%), Oxygen (4.96%), and Carbon (0%). The dentin bridge formed after induced by Nacre and Biodentine was significant (p<0.05). Biodentine is stronger in reducing dentin bridges than Nacre in the hard tissue formed of the initial dentinal bridge and complete dentin bridges. The Quantity level expression of TGF-β1 of dentine is higher than that influenced by Biodentine compared to the Nacre of all score categories. They are significant among the treatment of 7 days, 14 days, and 30 days (p<0.05). Nacre and Biodentine can support the healing of reversible pulpitis of Rattus novregicus, which is indicated by an increase in TGF-β1 expression in inducing the dentinal bridge formation of 7, 14, and 30 days.