1Department of Periodontology, Faculty of Dental MedicineUniversitas Airlangga, Jl. Prof. Dr. Moestopo No. 47Surabaya – Indonesia.
2Periodontology Specialist Program Student, Faculty of Dental MedicineUniversitas Airlangga, Jl. Prof. Dr. Moestopo No. 47Surabaya – Indonesia.
3Professor of Department of Periodontology, Faculty of Dental MedicineUniversitas Airlangga, Jl. Prof. Dr. Moestopo No. 47Surabaya – Indonesia.
*Corresponding Author E-mail: chiquita-p-s@fkg.unair.ac.id
Online Published on 23 February, 2023.
Damage to the periodontium tissue requires a regenerative treatment to increase the dimensions of the lost bone. This research was conducted to find another regenerative material with the use of type 1 collagen peptides derived from gourami fish scales. This study was conducted to test the viability of gourami scales collagen peptides on Human Gingival Fibroblast cells for 24 hours.
To determine the viability of Human Gingival Fibroblasts (HGF) after the administration of collagen peptides of gourami fish scales.
HGF was taken from healthy gingiva and planted in 96 well plates. The type 1 collagen peptides of gourami fish scales with concentrations of 0.32 mg / ml, 0.16 mg / ml, 0.04 mg / ml, 0.02 mg / ml and 0.01 mg / ml were inserted into each well and incubated for 24 hours. MTT Assay was performed to see the viability of fibroblast cells.
There was an increase in the viability value from a concentration of 0.32 mg/ml to 0.01 mg/ml. The concentration of 0.01 mg/ml showed the highest viability.
The collagen peptide is a potential substance for tissue engineering. The concentration of 0.01 mg/ml collagen peptides shows the highest HGF viability.
Regenerative, Collagen peptides, Viability, Human gingival fibroblast, Tissue engineering
