1Department of Chemistry, Kalasalingam Academy of Research and Education, Anand Nagar, Krishnankoil - 626126, Tamilnadu, India.
2Department of Humanities and Sciences, Vallurupalli Nageswara Rao Vignana Jyothi Institute of Engineering and Technology, Hyderabad, 500090, Telangana, India.
*Corresponding Author E-mail: gangadharaangajala@gmail.com
Online Published on 23 February, 2023.
The present study attempts to investigate the antioxidant activity and chromatographic profiling using HPLC and GC-MS technique of selected Indian Medicinal herbs. Evaluation of antioxidant activity of the methanolic extract of whole plant of Marsilea-quadrifolia (Sample 1), whole plant of Trigonella-foenum-graecum (sample 2), flowers of Cassia-auriculata (Sample 3), leaves of Mangifera-indica (sample 4), flower of Musa-paradisiaca (Sample 5), seeds of Eugenia-jambolana (sample 6), leaves of Aegle-marmelos (sample 7) and poly herbal Extract (PHE) with equal ratio of the mixture of the seven plants powder (Sample 8) were investigated by DPPH (2,2-diphenyl-1-picryl-hydrazyl) assay, Hydrogen peroxide assay and Nitric oxide scavenging activity. Percentage of inhibition and half-inhibition concentration, IC50 values were reported. GC-MS and HPLC analysis of PHE revealed the presence of various Chemical compounds which mainly includes polyphenols and flavanoids responsible for antioxidant activity.
PHE, HPLC, GC-MS, DPPH, IC50, Antioxidant
