Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2022
  • Volume: 15
  • Issue: 9

Collagen thickness and density in BALB/c mice exposed to UVB light after using siam weeds cream (Chromolaena odorata L.)

  • Author:
  • Karlina Amir Tahir1, Khairuddin Djawad2, Sartini Sartini3, Arief Budiyanto4, Ahmad Lalo5, Syatirah Jalaluddin6, Maulita Indrisari7, Syamsuri Syakri1, Asrul Ismail1, Khaerani1, Anshari Masri8, Upik A. Miskad9,*
  • Total Page Count: 6
  • Page Number: 4099 to 4104

1Department of Pharmacy, Faculty of Medicine and Health Science, UIN Alauddin Makassar, Makassar, Indonesia

2Department of Dermatology and Gender, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

3Department of Pharmacy and Science, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia

4Department of Dermatology and Gender, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia

5National Agency of Drug and Food Control, Makassar, Indonesia

6Faculty of Medicial and Health Science, UIN Alauddin Makassar, Makassar, Indonesia

7STIFA, Makassar, Indonesia

8Department of Pharmacy, Faculty of Medicine and Health Science, Universitas Muhammadiyah, Makassar, Indonesia

9Department of Anatomical Pathology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

*Corresponding Author E-mail: upik.miskad@gmail.com

Online published on 27 February, 2023.

Abstract

Ultraviolet B (UVB) radiation can cause damage to the skin's dermis layer, which can lead to collagen fragmentation. Damage to collagen generates wrinkles, which are a sign of aging.

The purpose of this study was to determine how Siam Weeds cream (Chromolaena odorata L.) altered the thickness and density of collagen in the skin of BALB/c mice after UVB exposure.

There were four groups of 24 BALB/c mice altogether: normal control, negative control (Cream Base with UVB), positive control (UVB), and treatment (C. odorata cream and UVB). Just before and after the mice were exposed to UVB at 500 mJ/cm2, the Siam Weeds cream formulation was applied at a concentration of 1%. Mice were slaughtered, and a back skin excisional biopsy was conducted to analyze collagen.

The treatment group differed significantly both in thickness and density of collagen against the positive control group and the negative control group (p<0.05) and There was no statistically significant difference between the treatment and normal control groups (p<0.05).

A 1% dosage of C. odorata cream can improve the thickness and density of collagen in UVB-exposed mice's skin.

Keywords

Chromolaena odorata L., Cream, Collagen, UVB