Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2022
  • Volume: 15
  • Issue: 9

Molecular analysis of bacteria isolated from the soil for its potential agnostic activity

Department of Computer Applications, PSGR Krishnammal College for Women, Peelamedu, Coimbatore - 641004, Tamil Nadu, India

*Corresponding Author E-mail: vinodhini.k@protonmail.com

Online published on 27 February, 2023.

Abstract

The work elucidates the agnostic action of a bacterial soil isolate, procured from different parts from Coimbatore, Tamilnadu, India. The bacteria strains were obtained via routine serial dilution plate protocols. The best isolate was tested against 3 pathogens; Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus. The screening was performed via streaking, and the isolate L1An1 exhibited agnostic activity on K pneumoniae and S aureus. The maximal impact of 21mm inhibition zone was recorded. Molecular identification and the resultant sequence were consigned for NCBI BLAST. The isolates L1An2 showed 99% resemblance with that of Enterobacter aerogenes. The sequence submitted to GenBank and MT192658.1 was procured the accession number.

Keywords

Agnostic action, Antimicrobial, Soil bacteria, Molecular identification