Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai - 116, Tamil Nadu, India
The phosphodiesterase (PDE)7B, belonging to family PDE7 has high affinity and specificity for cyclic adenosine monophosphate (cAMP). This was reported to regulate a range of physiological functions. Inhibition of this enzyme was found to have therapeutic effects. The present study aims to evaluate the inhibition of PDE7B by vasicine, using Nuclear magnetic resonance (NMR) spectroscopy.
The 1D NMR based time series experiment was performed; the initial and the final spectra were compared for the formation of cAMP in the presence and absence of the inhibitor.
The PDE7B was inhibited completely by vasicine and no formation of AMP was seen in the time series spectra.
NMR spectroscopy method revealed the PDE7B inhibitory activity of vasicine which may be considered as a therapeutic agent for diseases regulated by cAMP.
Cyclic AMP, Quinazoline alkaloid, NMR Spectroscopy, PDE7B, In-vitro
