Comparative Evaluation of HEP-2(Cincinnati) and Vero cell lines sensitivity to Bivalent Oral Polio Virus Vaccine (Type 1 and Type 3) by using In-vitro Microtitration Potency Assay

Poliomyelitis is a severe viral infectious disease caused by poliovirus and usually, children under the age of 5 years are affected mostly who are not immunized or fully vaccinated. For precise evaluation of quality control testing of the vaccine is based on three parameters i.e. Identity, efficacy and safety. One of the most important test is potency to know about the efficacy and well-founded results in quality control of vaccines.

To evaluate the and compare sensitivity of HEP-2(Cincinnati) and Vero cell lines to Bivalent Oral Polio Virus Vaccine (Type 1 and Type 3)

The sensitivity of Hep_2-c and Vero cell lines were compared by using a cell culture-based potency test based on CCID₅₀/SHD.

Present study showed that the Hep_2-C is most sensitive for bOPV (Type1 and Type3) and the Vero cell line is less sensitive for Type 3 Virus. The Vero cell line is similarly sensitive as Hep_2-c for Type1 virus and TVC (Total Virus Count) of bOPV.

The present study, suggest that the Vero cell line can also be used for the Potency assay of TVC (Total Virus Count) and Type1 in bivalent oral polio vaccine or monovalent (Type1) OPV along with Hep_2-c cell line.