Anti-inflammatory activity of Methanolic extract and Quercetin from the leave of Carica papaya Linn (Caricaceae)

To isolate bioactive compound from methanolic extract of Carica papaya leaves (MECPL) and investigate their anti-inflammatory potential.

Soxhlet extraction of C. papaya leaves powder was performed by using methanol as a solvent. The isolation of MECPL extract was performed by using column chromatography. All fractions obtained from column chromatography were subjected to HPLC, LCMS, FTIR, 1HNMR studies to identify and confirm isolated bioactive compound. The anti-inflammatory activity of isolated compound was performed by using Carrageenan induce models. Animals (Rats) of either sex were divided into six groups (n=6) at random. Group I-II control and negative control werereceived saline and carrageenan (1% w/v in 0.9% saline) respectively. Group III-VI were treated with 10, 150, 300 mg/kg and 10 mg/kg of isolated compound, MECPL and indomethacin respectively.

As a result, the compounds identified in the MECPL extract by HPLC, LCMS, FTIR, 1HNMR was Quercetin. The anti-inflammatory potential of MECPL and quercetin was investigated and Quercetin showed the best anti-inflammatory activity as compared to Indomethacin (standard) and MECPL. Conclusion: The present work used to identify and confirm the phenolic compounds (quercetin) in MECPL. It was suggested that this compound could be responsible to the anti-inflammatory activity, as reported by ethnomedicinal use of this plant. Through the experimental models performed, we can conclude that the results obtained with the MECPL support its potential use for the treatment of inflammatory disorders.