Hepatocorrective effects of Hydroethanolic Extract of Cocos nucifera L. sprout and Borassus flabellifer Sprout in Carbon tetrachloride (CCl₄) induced hepatotoxic rats

The objective of the study was to determine the hepatocorrective activity of hydroethanolic extract of Cocos nucifera L. and Borassus flabellifer sprout in carbon tetrachloride (CCl₄) induced hepatotoxic rats. The qualitative phytochemical analysis was used to evaluate the secondary metabolites like flavonoids, saponins, proteins in the hydroethanolic extract of Cocos nucifera L. sprout and Borassus flabellifer sprout which also reveals the presence of pharmacologically active phytochemicals. The toxicant CCl₄ was used to induce hepatotoxicity in 1 : 1 mixture with olive oil. Hydroethanolic extract of Cocos nucifera L. sprout and Borassus flabellifer sprout were administrated in the doses of 100mg/kg and 200mg/kg body weight orally for 21 days. Silymarin was used as a standard drug. The hepatocorrective effect of the extracts were evaluated by the assessment of biochemical parameters and liver markers such as Aspartate transaminase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP), Gamma glutamyl transferase (GGT), Lactate dehydrogenase (LDH), and also by the assessment of Total protein, Creatinine, Urea, Total cholesterol, Lipid peroxidation (LPO), Vitamin C, Superoxide dismutase (SOD), Catalase, Prothrombin Time (PT), Glutathione peroxidase (GPx), Reduced glutathione (GSH) etc. Assessment of enzymatic and nonenzymatic antioxidants and histopathological studies of liver were also done for the hepatocorrective activity determination. Treatment of rats with hydroethanolic extract of Cocos nucifera L. and Borassus flabellifer sprout significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The overall findings suggest that both sprouts have potent hepatocorrective activity in carbon tetrachloride induced hepatotoxic rats.