Isolation of betulinic acid from Byttneria herbacea (Malvaceae)

During the phytochemical analysis, flavonoids, saponins, tannins, glycosides, alkaloids, and terpenoids were identified in Byttneria herbacea (Malvaceae) among other things. The goal of this research is to identify and characterize the plant's bioactive principle. It has a wide range of folk medicinal applications. The gradient fractionation method was used to isolate and characterize phytoconstituents from ethanol extract. Physical, chemical, and spectroscopic data were used to determine the structure of the isolated substance (IR, UV, 1HNMR, MS). The ethanol extract of the plant yielded a triterpenoid structure. Various spectroscopical approaches were used to identify the odorless, colorless chemical that solubilizes in ethyl acetate and has a melting point of 315°C - 318°C and an R_f value of 0.62 in the mobile phase toluene: ethyl acetate (85:15). As a result of this research, the isolated component has been identified as Betulinic acid, which is accountable for the plant's diverse pharmacological properties.