1Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology, Lviv Polytechnic National University, Stepan Bandera Street, 12, Ukraine
2Danylo Halytsky Lviv National Medical University, Department of Microbiology, Lviv, 79013, Ukraine
3University of Opole, Faculty of Chemistry, Department Analytical Chemistry, Opole, Poland
4University of Opole, Department of Pharmacy and Ecological Chemistry, Opole, Poland
*Corresponding Author E-mail: viktoriia.r.liakh@lpnu.ua
Online Published on 12 October, 2023.
The analysis presented in this article is a continuation of a broader qualitative study examining the composition of the chloroform fraction of Caltha palustris. The study discovered 29 compounds, and 26 of them have been identified:paraffinic hydrocarbons, esters, fatty aldehydes, morphine derivatives, thiazole derivatives, benzodiazepines, naphthalene, and a small amount of terpene. It was figured out that the investigated lipophilic extract has an antioxidant impact. For the investigation, there were used such assays as the DPPH radical and the ABTS radical cation assays. Moreover the investigated lipophilic extract has an antimicrobial activity both in terms of gram-positive (Staphylococcus aureus (ATCC 25923 (F-49)), Bacillus cereus 34 (non-MDR), Enterococcus faecalis 26(MDR) and gram-negative, (Raoultella terrigena (ATCC 33257), Escherichia coli (ATCC 25922), Escherichia coli 168) microorganisms and yeast Candida spp. In addition, the significant content of biologically active substances in the lipophilic volatile fraction of Caltha palustris indicates the prospects for further study.
Caltha palustris, Chloroform fraction, Gas chromatography, Lipophilic extract, Antioxidant effect, Antimicrobial activity
