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*Corresponding Author E-mail: rekha.kini@manipal.edu
Bisphenol A (BPA) is considered one of the most common industrial manufactured chemicals. Vitamin C is an important dietary antioxidant which significantly decreases the adverse effects of reactive oxygen species.
To evaluate the damaging effect of Bisphenol on testes and the role of vitamin C on Bisphenol induced testicular damage.
Institutional ethics committee clearance was obtained before beginning the experiments. Animals were divided into the following groups with 6 rats in each group; Group 1: Control Group (Olive oil treated) Group 2: (Vitamin C treated), Group 3: (Bispenol treated), Group 4: (Vitamin C + Bispenol treated). Testicular tissue was processed to measure MDA and GSH level, sperm count and sperm morphology, histology. Serum level of testosterone was estimated.
Results of the present study showed a significant decrease in the level of GSH and sperm count and testosterone level and increase in MDA in Group 3 compared to GR.1and GR.2. Pre-treatment with Vitamin C showed an increase in the level of GSH and testosterone as well as sperm count compared to Bisphenol treated rats. Histological results were supported the biochemical results. Vitamin C also showed decrease in sperm shape abnormality which was induced by Bisphenol intoxication.
Thus we have hypothesized that vitamin C could act as an antioxidant against BPA induced oxidative stress.
Bisphenol, Oxidative stress, Testosterone, Vitamin C