1Tutor, Department of Physiology, KVG Medical College and Hospital, Sullia University, Deralakatte, Mangalore
2Department of Physiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India
3Department of Urology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India
4Department of Biochemistry, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India
*Corresponding Author E-mail: rekha.kini@manipal.edu
Online Published on 16 October, 2023.
Bisphenol A (BPA) is considered one of the most common industrial manufactured chemicals. Vitamin C is an important dietary antioxidant which significantly decreases the adverse effects of reactive oxygen species.
To evaluate the damaging effect of Bisphenol on testes and the role of vitamin C on Bisphenol induced testicular damage.
Institutional ethics committee clearance was obtained before beginning the experiments. Animals were divided into the following groups with 6 rats in each group; Group 1: Control Group (Olive oil treated) Group 2: (Vitamin C treated), Group 3: (Bispenol treated), Group 4: (Vitamin C + Bispenol treated). Testicular tissue was processed to measure MDA and GSH level, sperm count and sperm morphology, histology. Serum level of testosterone was estimated.
Results of the present study showed a significant decrease in the level of GSH and sperm count and testosterone level and increase in MDA in Group 3 compared to GR.1and GR.2. Pre-treatment with Vitamin C showed an increase in the level of GSH and testosterone as well as sperm count compared to Bisphenol treated rats. Histological results were supported the biochemical results. Vitamin C also showed decrease in sperm shape abnormality which was induced by Bisphenol intoxication.
Thus we have hypothesized that vitamin C could act as an antioxidant against BPA induced oxidative stress.
Bisphenol, Oxidative stress, Testosterone, Vitamin C
