Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2023
  • Volume: 16
  • Issue: 5

Effect of aqueous extract of Trigonellafoenum-graecum L. seeds on Acetic acid- induced Ulcerative colitis in rats

  • Author:
  • Aqsa Fathima1, Shivaprakash Gangachannaiah1,*, Ujjal Bose2, Shama Prasada Kabekkodu3, Rituparna Chakraborty4, Praveen S E Kumar5, Padmanabha Udupa6, Rachagolla Sai Prathap Yadav7, Vidya Monappa8
  • Total Page Count: 8
  • Page Number: 2161 to 2168

1Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104

2Department of Pharmacology, American University of AntiguaCollege of Medicine, Coolidge, AntiguaP.O. Box 1451

3Department of Cell and Molecular biology, Manipal School of Life Sciences, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104

4Department of Microbiology, Melaka Manipal Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104

5Department of Pharmacology, Manipal-Tata Medical CollegeJamshedpur, Manipal Academy of Higher Education, Jharkhand, India - 831017

6Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104

7Centre for Molecular Neuroscience, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104

8Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India - 576104

*Corresponding Author E-mail: shiva.g@manipal.edu

Online Published on 16 October, 2023.

Abstract

Ulcerative colitis (UC) is an inflammatory bowel disorder affecting the colonic mucosa, characterized by intense inflammation and mucosal damage. The present study aimed to evaluate the protective effect of the Trigonellafoenum-graecum L. (TFG) seeds in acetic acid-induced UC in rats.

Male rats (n=30) were distributed into 5 groups as normal control, UC, standard, and two test groups. Colitis was induced by acetic acid in all the groups except the normal control group. Normal control and UC group received distilled water, the standard group was administered sulfasalazine at 100mg/kg body weight (bw), and test groups, TFG-I, and TFG-II received TFG seed extract at 500 and 1000 mg/kg bw, respectively. The duration of treatment was 7 days, and colitis was induced on day 8. Animals were sacrificed on day 9 and colonic tissue was dissected and collected for biochemical, molecular, and histological analysis.

The disease activity index score in standard, TFG-I, and TFG-II (3.33±0.21, 2.66±0.21, and 3.50±0.22) was significantly lesser (P<0.05) than scores in the UC group (4±0.01). The macroscopic score indicating the intensity of mucosal inflammation was significantly decreased (P&le; 0.01) to4.0±0.25, 3.16±0.30, and 3.83±0.40 in standard, TFG-I, and TFG-II groups, respectively compared to the UC group (4.66±0.21). Similarly, there was a significant reduction (P&le;0.05) in histological scores of the standard, TFG-I, TFG-II (3.5±0.34, 1.25±0.34, 3.25±0.34) groups compared to the UC group (4.75±0.34). Biochemical assessment in the standard and test groups showed significant increase (P<0.05) in total protein, reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) levels whereas significant reduction (P<0.01) in malondialdehyde (MDA) levels compared to UC group. The pro-inflammatory cytokine TNF-α levels were significantly decreased (P<0.01) in standard, TFG-I, and TFG-II (−1.75±0.007, -0.27±0.17 and -0.51±0.002) when compared to the UC group (0.20±0.02).

The study demonstrates the ability of TFG seeds in reducing the inflammatory and oxidative stress induced mucosal damage in acetic acid-induced UC in rats.

Keywords

Inflammatory bowel disorder, Ulcerative colitis, Trigonellafoenum-graecum L, Acetic acid, TNF-α