Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2023
  • Volume: 16
  • Issue: 5

Derivative spectroscopic method and RP-HPLC method development and validation of levofloxacin hemihydrate

  • Author:
  • Sandip Sen1,*, Ravindar Bairam2, Sirikonda Jala2, Laxmi Dharabonia2, Rajeshwari Konika2
  • Total Page Count: 6
  • Page Number: 2239 to 2244

1Department of Pharmaceutical Chemistry, School of Pharmacy, Guru Nanak Institutions Technical Campus, Ibrahimpatnam, Hyderabad - 501506

2Department of Pharmaceutical Chemistry, Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Kondapakka, Siddipet - 502277

*Corresponding Author E-mail: sandipsen2010@gmail.com

Online Published on 16 October, 2023.

Abstract

The present work describes the development of a simple, precise and accurate derivative spectroscopic and RP-HPLC method for the estimation of levofloxacin hemihydrate in the tablet dosage form. Levofloxacin hemihydrate showed absorption maxima at 287.36, 287.2 and 296.2nm in zero, first and second-order spectroscopic methods. The RP-HPLC method was optimized by using Orthophosphoric acid: acetonitrile: methanol (50: 20: 30) as solvent at 287nm. The methods showed linearity in the concentration range 1-5 and 2-10 μg/ ml and the correlation coefficient was 0.999 respectively. The method has been validated according to the ICH guidelines. The percentage relative standard deviation was found to be less than 2% in respect of both methods.

Keywords

Levofloxacine hemihydrates, Derivative spectroscopic method, RP-HPLC method, ICH guidelines