Selection of an active association of probiotic bacteria and the optimal composition of the nutrient medium for cultivation to increase the therapeutic and prophylactic effectiveness of a medicinal probiotic preparation against intestinal infections

The study aimed to create associations of probiotic bacteria with a wide range of biological activity, resistance to antibiotics, and selection of the nutrient medium composition to increase the effectiveness of probiotics against intestinal infections in humans. The authors found that the tested associations of probiotic bacteria exhibited sufficient antagonistic activity to all test cultures taken in the experiment. Associations No. 2 on media 1 and 4, No. 5 on media 1 and 8, and association No. 9 on media 1 and 8 showed the greatest activity for most test cultures. These associations have a bacterial titer of nx 10¹⁰ colony-forming units/ml, contain proteolytic, amylolytic, and pectolytic enzymes, as well as B vitamins, such as B₃, B₅, B₆, B₈, and B₁₂. The authors studied the resistance of selected associations of lactic acid and propionic acid bacteria to the antibiotics used, which allows these associations to be used in complex therapy if necessary. Selected nutrient media and active associations of probiotic bacteria with a wide range of biological activity and antibiotic resistance can be used to create effective targeted medicinal probiotic agents.