1GITAM School of Pharmacy, GITAM Deemed to be University, Visakhapatnam, India-530045
2College of Pharmaceutical Sciences, Department of Pharmaceutical Biotechnology, Andhra University, Visakhapatnam, Andhra Pradesh, 530 003, India
3Raghu College of Pharmacy, Dakammari, Bheemunipatnam, Visakhapatnam, Andhra Pradesh, 531162, India
*Corresponding Author E-mail: mary.pharma114@gmail.com
Online Published on 31 October, 2023.
Mangroves are found to be an excellent source for the isolation of novel bioactive metabolite producing microorganisms. Streptomyces hydrogenans KMFA-1, isolated from mangrove soil sample is exclusively known for its potent activity against various pathogenic fungi. The present study is focused on strain improvement of Streptomyces hydrogenans KMFA-1 by physical and chemical mutagens such as ultraviolet irradiation and nitrous acid (HNO2) respectively, for enhancing the production of antifungal metabolite against C. albicans and Pectinotrichum llanense. The parent isolate KMF-A1 produced 20mm inhibition zone diameter of antifungal activity while, the UV mutant designated as UV7, yielded 28mm and the nitrous acid mutant. UV7N4 (KMF-A1) exhibited 30mm inhibition zone diameter. The mutant strain produced improved antifungal activity by 1.5-fold increase with respect to wild strain.
Streptomyces hydrogenans, Strain improvement, Candida albicans, Mutagens, Antifungal metabolite
