GITAM School of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, India
*Corresponding Author E-mail: mmukthin@gitam.edu
Online Published on 31 October, 2023.
Rilpivirine is a new second-generation non-nucleoside reverse transcriptase inhibitor used as an anti-viral drug. A new stability indicating reverse phase ultrafast liquid chromatographic method was developed for the assay of Rilpivirine in tablet dosage forms. A Shimadzu UFLC system equipped with Phenomenex C8 column and PDA detector was employed for the chromatographic study. Mobile phase mixture consisting of 10 mM tetra butyl ammonium hydrogen sulphate: Acetonitrile (55: 45) and flow rate 1.0 mL/min (UV detection at 280 nm). Beer-Lambert’s law was obeyed over a concentration range 0.05-40 μg/mL. The linear regression equation was found to be y = 254045x + 11208 (r2 = 0.9999). The LOD and LOQ was found to be and 0.0136 and 0.0414 μg/mL respectively. Stress degradation studies were performed and the method was validated as per ICH guidelines. The proposed method is simple, precise, accurate and robust and can be applied for the quantification of Rilpivirine tablet dosage forms.
Reverse phase Ultrafast liquid Chromatographic method, Stress degradation
