1Magister Program in Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia
2Department of Nanotechnology Engineering, Faculty of Advance Technology and Multidiscipline, Universitas Airlangga, Surabaya, 60115, Indonesia
3Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya-60115, Indonesia
4Institute of Analytical and Environmental Sciences, National Tsing Hua University, Sec. 2 Kuang Fu Road, Hsinchu30013, Taiwan
5Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, 60115, Indonesia
6Institute of Science Technology and Health, Jombang61419, Indonesia
*Corresponding Author E-mail: windarmanto@fst.unair.ac.id
Online Published on 2 November, 2023.
Polysaccharide extract has potential as an anticancer agent. Abelmoschus esculentus is one of the plants that is rich in polysaccharide content, but its anticancer activity is still need explore. This study aimed to explore the anticancer activity of Abelmoschus esculentus crude extract polysaccharide by measure the effect on levels of pro-inflammatory cytokines IL-2, IL-12, TNF-α, IFN-γ in HeLa cell line. This study used HeLa cells as a research model with four groups, including KN (media and DMSO), K+ (doxorobicin μg/mL), P1 (Abelmoschus esculentus L. extract 200μg/mL) and P2 (Abelmoschus esculentus L. extract 400μg/mL). The Abelmoschus esculentus L. was extracted using boiled distilled water. Cytokine levels were analyzed using the ELISA test. The results of cytokine levels showed decreasing in the levels of cytokines IL-2, IL-12, TNF-α, IFN-γ, (P < 0.005). In conclusion, the polysaccharide extract of Abelmoschus esculentus L. has the potential as an anticancer and therapeutic agent for cancer. Research on green okra polysaccharide crude extract as an immunosuppressant is expected to be a potential bio-pharmaceutical source.
Polysaccharide, Abelmoschus esculentus, Cancer, HeLa cell, Healthy life
