HPTLC method development of herbal drugs and its validation: An overview

High Performance Thin Layer Chromatography (HPTLC) is the most potent and sophisticated type of Thin Layer Chromatography (TLC). It uses chromatographic layers with the highest levels of separation, efficiency and employs high-tech equipment for every step of the process, including accurate sample application, standardised reproducible chromatogram development, and software-controlled evaluation. HPTLC is a concept that incorporates both the use of established methodologies for qualitative and quantitative analysis and a widely standardised methodology founded on scientific facts. The resolution can be increased and more exact quantitative measurements, which satisfies all quality standards for today's analytical needs. Development of an analytical technique based on HPTLC and parameter validation in line with practical assessment. It complies with standards while reducing mistakes and inquiries. Quality Control and Quality Assurance of raw materials of Plant Origin can easily and effectively be done qualitative characterization and quantitative determination of mixtures of substances, Chemical Fingerprinting by High Performance Liquid Chromatography. This review article provides fundamental principles, guidance for proper validation practise, aids in selecting the best mobile phase, and clarifies the processes of the analytical process., protocol, separation, resolution, validation process, current advancements, changes made to TLC that led to HPTLC, optimization, process control, automation, and hyphenation.